Recombinant Mesothelin 抗体
Quick Overview for Recombinant Mesothelin 抗体 (ABIN7543155)
抗原
See all Mesothelin (MSLN) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Mesothelin [K1], Mouse IgG1, kappa
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特异性
- This antibody can bind the human mesothelium, expressed on many cancer cells like ovarian carcinoma.
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交叉反应
- Cynomolgus
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产品特性
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Original Species of Ab: Mouse
Original Format of Ab: IgG1
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunization of mice with periodate-treated human ovarian cancinoma (OVCAR-3) cells. These mice had previously been made tolerant to normal human kidney membranes.
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亚型
- IgG1 kappa
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anti-Mesothelin (MSLN) (AA 273-407) antibody
MSLN 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal MSLN-2131 unconjugated
anti-Mesothelin (MSLN) (AA 101-200) antibodyMSLN 适用: 人 ELISA, WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) (AA 273-407) antibodyMSLN 适用: 人 IHC (f) 宿主: 小鼠 Monoclonal unconjugated
anti-Mesothelin (MSLN) (AA 37-286) antibodyMSLN 适用: 人, 猴 ELISA, WB, FACS 宿主: 小鼠 Monoclonal 5C11A4 unconjugated
anti-Mesothelin (MSLN) (AA 37-598) antibodyMSLN 适用: 人 ELISA, IHC, IP, IF 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) (AA 296-580) antibodyMSLN 适用: 人 ELISA, FACS 宿主: 兔 Monoclonal DM71 unconjugated Recombinant Antibody
anti-Mesothelin (MSLN) (AA 296-606) antibodyMSLN 适用: 人, 大鼠 ELISA, WB 宿主: 小鼠 Monoclonal 7E6A6 unconjugated
anti-Mesothelin (MSLN) (AA 37-286) antibodyMSLN 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4H12F8 unconjugated
anti-Mesothelin (MSLN) (AA 1-62) antibodyMSLN 适用: 人 ELISA, WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mesothelin (MSLN) antibodyMSLN 适用: 人, 小鼠, 大鼠 IF 宿主: 兔 Polyclonal unconjugated
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应用备注
- This antibody was used for the study and characterization of CAK1 antigen present on ovarian cancers and normal mesothelium. The initial screening of the mesothelin reactive clones to live OVCAR-3 cells was done using immunofluorescence. Secondary screening was done using immunoperoxidase histochemistry on cryostat sections of normal human tissues and human tumors. Quantitative ELISA confirmed that K1 and OC125 antibodies bind to different epitopes on the OVCAR-3 cells (PMID: 1735605). This antibody can detect mesothelin in a western blot assay. Competition RIA was used to detect the presence of CAK1 antigen in the supernatent of protease or phosphatidylinositol-phospholipase C treated cells. An immunotoxin composed of K1 antibody and native Pseudomonas exotoxin was found to be selectively cytotoxic to cells expressing CAK1 antigen (PMID: 1727378). The affinity of K1 antibody to mesothelin was Kd= 8 nM and determined using surface plasmon resonance (BIAcore assay) (PMID: 9178820).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Mesothelin (MSLN)
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别名
- Mesothelin
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背景
- MSLN, CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor
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UniProt
- Q13421
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途径
- EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
抗原
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