Recombinant CD11b 抗体
Quick Overview for Recombinant CD11b 抗体 (ABIN7543092)
抗原
See all CD11b (ITGAM) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD11b [M1/70], Rabbit IgG, kappa
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特异性
- This antibody reacts with mouse CD11b, the 165-170 kDa integrin alphaM. CD11b non-covalently associates with CD18 to form alphaMbeta2 integrin (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen. The Mac-1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, and blood monocytes. CD11b is expressed on 8 % of spleen cells, 44 % of bone marrow cells, and less than 1 % of thymocytes, and is commonly used as a microglial marker in nervous tissue.
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产品特性
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Original Species of Ab: Rat
Original Format of Ab: IgG2b
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was raised by immunising DA (dark agouti) and Albino Oxford (AO) strain inbred rats with nylon wool-purified B10 mouse spleen cells according to the protocol by Springer et al. (Eur J Immunol. 1978).
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亚型
- IgG kappa
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anti-Integrin alpha M (ITGAM) (AA 520-570) antibody
ITGAM 适用: 人, 小鼠, 大鼠 FACS 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 145-340) antibodyITGAM 适用: 人 IHC, WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 77-180) antibodyITGAM 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (fro), ELISA, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 253-282), (N-Term) antibodyITGAM 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB17991 unconjugated
anti-Integrin alpha M (ITGAM) (AA 31-160) antibodyITGAM 适用: 大鼠 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Integrin alpha M (ITGAM) (AA 941-1074) antibodyITGAM 适用: 人 IHC, WB, StM 宿主: 小鼠 Monoclonal ITGAM-3338 unconjugated
anti-Integrin alpha M (ITGAM) (AA 941-1074) antibodyITGAM 适用: 人 IHC, WB, StM 宿主: 小鼠 Monoclonal ITGAM-3340 unconjugated
anti-Integrin alpha M (ITGAM) antibody (FITC)ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 FITC
anti-Integrin alpha M (ITGAM) antibody (Biotin)ITGAM 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-174 Biotin
anti-Integrin alpha M (ITGAM) antibody (PE)ITGAM 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-174 PE
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应用备注
- This antibody has been used in FACS analyses, ELISA, immunoprecipitation, immunoblotting, immunohistochemistry (frozen/paraffin), immunocytochemistry, neutralisation and functional assays in numerous studies, for example, to demonstrate that the Hippo, Wnt/beta-catenin, and Notch pathways interact to regulate liver size and inhibit hepatocellular carcinoma (Kim et al., 2017), to understand the role of PD-1 in maintaining islet-specific tolerance in diabetes-resistant strains (Martinov et al., 2016), to examine the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al., 2015), to demonstrate that the small intestinal gut-associated lymphoid tissues (GALT) are the major early sites of prion accumulation and neuroinvasion after oral exposure in oral prion disease pathogenesis (Donaldson et al., 2015), to evaluate the role of Wnt signalling in the ageing of haematopoietic stem cells in mice (Florian et al., 2013), to investigate the relative contribution of receptors for advanced glycation end products (RAGE)-dependent and RAGE-independent signalling pathways in diabetic nephropathy (Watson et al., 2012), to dissect the role of CD11b in obesity-associated inflammation (Zheng et al., 2015), and to describe a novel approach to efficiently differentiate large numbers of mature inflammatory monocytes from heterogeneous bone marrow cell suspensions (Francke et al., 2011).
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说明
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This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD11b (ITGAM) (Integrin alpha M (ITGAM))
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别名
- CD11b
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背景
- Mac-1 alpha, CD11 antigen-like family member B, CD11B (p170), cell surface glycoprotein MAC-1 alpha subunit, cell surface glycoprotein MAC-1 subunit alpha, complement component receptor 3 alpha-a, complement receptor type 3, CR-3 alpha chain, integrin alpha-M, leukocyte adhesion receptor MO1, macrophage antigen alpha
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UniProt
- A1E2I0
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途径
- Apoptosis, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
抗原
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