Recombinant CD4 抗体
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北京 101111
Quick Overview for Recombinant CD4 抗体 (ABIN7543050)
抗原
See all CD4 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD4 [OX-68], Mouse IgG2a, kappa
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特异性
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This antibody is reactive with rat CD4 domain 3 & 4, and is partly competitive with OX-67 & 69.
Application Notes original Clone: This antibody binds to rat CD4. Upon challenge with HIV-1, this antibody was able to significantly inhibit viral entry into HeLa cells expressing a mutant of rat CD4 that binds gp120 (SImon, 1997). Similarly, pre-binding of gp120 resulted in a 50 % reduction in binding of this antibody, indicating epitope masking. Analogous effects on viral entry and synctium formation are observed with MAbs against human CD4.
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纯化方法
- Protein A affinity purified
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免疫原
- This antibody was prepared by subcutaneously injecting BALB/c mice with a soluble form of rat CD4, consisting of domains 3 and 4. A boost of a mixture of free sCD4 and sCD4 bound to cells was administered intravenously.
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亚型
- IgG2a, kappa
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anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人 FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠, 猴, 小鸡, 犬, 兔, Cow, 绵羊, 豚鼠, 山羊, Pig, 马, Avian, 驴, Hamster WB, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated
anti-CD4 (CD4) (AA 231-330) antibodyCD4 适用: 小鼠 FACS, WB, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) (N-Term) antibodyCD4 适用: 人 FACS, WB, MS 宿主: 小鼠 Monoclonal MEM-241 unconjugated
anti-CD4 (CD4) antibodyCD4 适用: 人, 小鼠, 大鼠 IHC 宿主: 小鼠 Monoclonal unconjugated
anti-CD4 (CD4) (AA 301-400) antibodyCD4 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD4 (CD4) antibody (PE)CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE
anti-CD4 (CD4) antibodyCD4 适用: 小鼠 FACS, IP, ICC, IHC (fro), Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated
anti-CD4 (CD4) (N-Term) antibody (FITC)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC
anti-CD4 (CD4) (N-Term) antibody (PE)CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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OriginalSpeciesName: Mouse
OriginalFormat: IgG2a
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- CD4
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别名
- CD4
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背景
- Leu3/T4, T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, W3/25 antigen
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UniProt
- P05540
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途径
- TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
抗原
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