Recombinant Transferrin Receptor 抗体
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Quick Overview for Recombinant Transferrin Receptor 抗体 (ABIN7543001)
抗原
See all Transferrin Receptor (TFRC) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-CD71 [OX-26], Rabbit IgG, kappa
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特异性
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This antibody is specific for the rat transferrin receptor, also known as CD71.
Application Notes original Clone: The original format of this antibody (mouse IgG2a) was developed and demonstrated to effectively labele transferrin receptor-expressing cells, including dividing lymphocytes and erythroid precursors, with minimal binding to non-dividing lymphoid cells or mature erythrocytes. It was used for fluorescence-based cell sorting to isolate subpopulations of rat hematopoietic stem and progenitor cells from bone marrow and fetal liver (Jefferies et al., 1985, PMID: 2981766). The original format of the antibody was employed in immunohistochemical staining of cryostat sections from rat brain. In vivo testing in PVG/c rats involved injecting the antibody intravenously, followed by cryostat sectioning and staining. The results showed clear labelling of rat brain capillaries, especially in the cerebellum and cortex, with weaker labelling after 16 hours. No such labelling was observed in other rat tissues, supporting the specificity for brain capillaries (Jefferies et al., 1984, PMID: 6095085). This antibody was utilized as a drug carrier to deliver compounds across the blood-brain barrier (BBB) in vivo in Sprague-Dawley rats. The antibody demonstrated selective, dose-dependent binding to brain capillary endothelial cells when administered intravenously, with localization observed as early as 5 minutes post-injection and evidence of transcytosis into the brain parenchyma over time. When conjugated with methotrexate (MTX), the antibody retained its targeting capabilities, facilitating significant accumulation of the drug in the brain parenchyma compared to free MTX (Friden et al., 1991, PMID: 2052557). This antibody was used to deliver nerve growth factor (NGF) across the BBB in in vivo experiments with aged Fischer 344 rats. The OX-26-NGF conjugate was administered intravenously and demonstrated significant therapeutic effects, including enhanced cholinergic markers in grafted tissue, improvement in spatial learning in aged rats, and upregulation of NGF receptors (Granholm et al., 1998, PMID: 9683326). Mutational screening showed that this antibody's crossing of the BBB is dependent on the its affinity to the receptor (Thom et al., 2018, PMID: 29485883). The antibody can also be used in flow cytometry, immunofluorescence, and western blot, and it may also be used in frozen-section IHC.
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产品特性
- This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
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纯化方法
- Protein A affinity purified
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免疫原
- The original antibody was generated by immunizing BALB/c mice with phytohaemagglutinin (PHA)-activated PVG rat lymph node cells with NS 1/1.Ag 4.1 myeloma cells.
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亚型
- IgG, kappa
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anti-Transferrin Receptor (p90, CD71) (TFRC) antibody
TFRC 适用: 人 FACS, IP, ICC 宿主: 小鼠 Monoclonal MEM-75 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) antibodyTFRC 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 578-765) antibodyTFRC 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 1-140) antibodyTFRC 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 1-198) antibodyTFRC 适用: 人, 小鼠, 大鼠 FACS, WB, IHC, IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 68-168) antibodyTFRC 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1E6 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 608-727) antibodyTFRC 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA 宿主: 小鼠 Monoclonal 1A1B2 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) antibody (Biotin)TFRC 适用: 人 FACS, IP, ICC 宿主: 小鼠 Monoclonal MEM-75 Biotin
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 649-677), (C-Term) antibodyTFRC 适用: 人 FACS, WB, IHC (p) 宿主: 兔 Polyclonal RB20736 unconjugated
anti-Transferrin Receptor (p90, CD71) (TFRC) (AA 621-720) antibodyTFRC 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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OriginalSpeciesName: Mouse
OriginalFormat: IgG2a
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))
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别名
- CD71
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背景
- Transferrin receptor, TfR, p90, T9, TFR1, OX26, MRC OX26, MRC OX-26
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UniProt
- Q99376
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途径
- Transition Metal Ion Homeostasis
抗原
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