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UCHL1 抗体

Rockland 900-301-e52 UCHL1 适用: 人 WB, IHC, ELISA, FM 宿主: 小鼠 Monoclonal BH7 unconjugated
Rockland
产品编号 ABIN7540657
Supplier Product No.: 900-301-e52
发货至: 中国
  • 抗原 See all UCHL1 抗体
    UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
    适用
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    宿主
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    小鼠
    克隆类型
    • 100
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    单克隆
    标记
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    This UCHL1 antibody is un-conjugated
    应用范围
    • 145
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    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Fluorescence Microscopy (FM)
    Supplier Product No.
    900-301-e52
    Supplier
    Rockland
    原理
    Ubiquitin Hydrolase (UCHL1) Antibody
    交叉反应 (详细)
    Anti-UCHL1 antibody is directed against human UCHL1 .
    纯化方法
    The antibody is protein G purified from concentrated clarified cell culture supernate.
    免疫原
    Anti-UCHL1 Monoclonal Antibody was produced in mouse by repeated immunizations with recombinant full length human UCHL1 extensively purified from E. coli.
    克隆位点
    BH7
    亚型
    IgG1
    Top Product
    Discover our top product UCHL1 Primary Antibody
  • 应用备注

    ELISA_Dilution: 1:10,000

    IF_Microscopy_Dilution: 1:500

    Western_Blot_Dilution: 1:5000

    说明

    Anti-Ubiquitin Hydrolase antibody is tested for use in ELISA and Western Blot, IHC, ICC, and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 24 kDa in size corresponding to the UCHL1 proteins in Western blot in the appropriate cell lysate or extract.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 50 % (v/v) Glycerol

    Preservative: 0.01 % (w/v) Sodium Azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at -20° C prior to opening.  This product is stable at 4° C as an undiluted liquid.  For extended storage, aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
    有效期
    12 months
  • 抗原
    UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
    别名
    UCHL1 (UCHL1 产品)
    背景
    Mouse anti-UCHL1 Antibody, mouse anti-Ubiquitin Hydrolase Antibody, UCH-L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1,UCHL1 antibody recognizes Ubiquitin C-terminal hydrolase 1 (UCHL1) which is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5. It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein. The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice. Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease. Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker. Therefore, UCHL1 monoclonal antibody is ideal for investigators involved in Neuroscience.
    基因ID
    7345
    UniProt
    P09936
    途径
    Feeding Behaviour
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