LC3B 抗体 (Internal Region)
Quick Overview for LC3B 抗体 (Internal Region) (ABIN7540630)
抗原
See all LC3B (MAP1LC3B) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-mn5
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Supplier
- Rockland
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原理
- LC3B Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human LC3B.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-LC3B antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Microtubule-associated proteins 1A/1B light chain 3B conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibody
MAP1LC3B 适用: 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibodyKO Validated MAP1LC3B 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibodyMAP1LC3B 适用: 人, 小鼠, 大鼠 WB, IHC (p), FACS 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibodyMAP1LC3B 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5H12 unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 74-106) antibodyMAP1LC3B 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 1-30), (N-Term) antibodyMAP1LC3B 适用: 人, 大鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (N-Term) antibodyMAP1LC3B 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) antibodyMAP1LC3B 适用: 人 WB, IF 宿主: 小鼠 Monoclonal unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 89-122), (cleaved) antibodyMAP1LC3B 适用: 人, 小鼠 IF, ICC 宿主: 兔 Polyclonal RB15839-RB28608 unconjugated
anti-Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B) (AA 77-106) antibodyMAP1LC3B 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: 1:100
Flow_Cytometry_Dilution: 1:25
Western_Blot_Dilution: 1:1000
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说明
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Anti-LC3B Antibody has been tested in ELISA, Western Blot, Immunohistochemistry, and Flow Cytometry. Positive control used U251-MG Cells in Flow and Human lymph node in IHC. Testing only recommended in WB when using recombinant or overexpressing systems. Expect a band at ~14.6 kDa in western blot using appropriate lysates or tissues.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))
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别名
- MAP1LC3B
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背景
- Rabbit Anti-Microtubule Associated Protein 1 Light Chain 3 Beta Antibody, Rabbit Anti-LC3B Antibody, Microtubule-Associated Proteins 1A/1B Light Chain 3B, Autophagy-Related Ubiquitin-Like Modifier LC3 B, MAP1 Light Chain 3-Like Protein 2, MAP1A/MAP1B Light Chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-Associated Protein 1 Light Chain 3 Beta, Autophagy-Related Protein LC3 B, MAP1A/1BLC3, MAP1LC3B-A, MAP1ALC3, ATG8F, LC3B,MAP1LC3B (Microtubule Associated Protein 1 Light Chain 3 Beta) is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. It is a ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). LC3B plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Anti-LC3B Antibody is useful for researchers interested in spastic paraparesis, neuroendocrine system, neuroscience research, cancer research and apoptosis research.
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基因ID
- 81631
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NCBI登录号
- NP_073729
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UniProt
- Q9GZQ8
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途径
- Autophagy
抗原
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