SNAIL 抗体 (Internal Region)
Quick Overview for SNAIL 抗体 (Internal Region) (ABIN7540622)
抗原
See all SNAIL (SNAI1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-mm5s
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Supplier
- Rockland
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原理
- SNAI1 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human SNAI1.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-SNAI1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human SNAI1 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-SNAIL (SNAI1) (AA 1-100) antibody
SNAI1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SNAIL (SNAI1) (C-Term) antibodySNAI1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SNAIL (SNAI1) (C-Term) antibodySNAI1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SNAIL (SNAI1) (N-Term) antibodySNAI1 适用: 人, 小鼠, 大鼠, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SNAIL (SNAI1) (AA 121-230) antibodySNAI1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2G11 unconjugated
anti-SNAIL (SNAI1) (AA 1-264) antibodySNAI1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-SNAIL (SNAI1) (AA 9-39), (N-Term) antibodySNAI1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal RB01400 unconjugated
anti-SNAIL (SNAI1) antibodySNAI1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-SNAIL (SNAI1) antibodySNAI1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SNAIL (SNAI1) antibodySNAI1 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000-1:50,000
Immunohistochemistry_Dilution: 1:300
IF_Microscopy_Dilution: 15 μg/mL
Western_Blot_Dilution: 1:2000
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说明
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Suggested Applications: User Optimized
Anti-SNAI1 Antibody has been tested in ELISA, WB, IHC, and IF. Expect a band at ~29 in western blot using appropriate lysates. Positive control used: SNAI1 overexpressed HEK293 in WB, human lung tissue in IHC, NIH-3T3 and HeLa in IF. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- SNAIL (SNAI1)
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别名
- SNAI1
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背景
- Rabbit Anti-SNAI1 Antibody, Snail Family Transcriptional Repressor 1, Snail Family Zinc Finger 1, Zinc Finger Protein SNAI1, Protein Snail Homolog 1, Protein Sna, SNAH, Snail 1 (Drosophila Homolog), Zinc Finger Protein, Snail Homolog 1 (Drosophila), Snail 1 Zinc Finger Protein, Snail 1 Homolog, Snail Homolog 1, DJ710H13.1, SLUGH2, SNAIL1, SNAIL, SNA,SNAI1 (Snail Family Transcriptional Repressor 1) is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. SNAI1 is involved in the induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. It binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. During EMT, SNAI1 is involved with LOXL2 in negatively regulating pericentromeric heterochromatin transcription (By similarity). SNAI1 recruits LOXL2 to pericentromeric regions to oxidize histone H3 and repress transcription which leads to release of heterochromatin component CBX5/HP1A, enabling chromatin reorganization and acquisition of mesenchymal traits (By similarity). It associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, SNAI1 may also activate the CDKN2B promoter by itself. SNAI1 may play a role in Gastroesophageal Junction Adenocarcinoma and Fasciolopsiasis. Anti-SNAI1 Antibody is useful for researchers interested in Cytoskeleton remodeling Regulation of actin cytoskeleton by Rho GTPases and Kit receptor signaling pathways.
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基因ID
- 6615
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NCBI登录号
- NP_005976
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UniProt
- O95863
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途径
- Negative Regulation of intrinsic apoptotic Signaling
抗原
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