MECP2 抗体 (C-Term)
Quick Overview for MECP2 抗体 (C-Term) (ABIN7540619)
抗原
See all MECP2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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Supplier Product No.
- 600-401-mm0
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Supplier
- Rockland
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原理
- MeCP2 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human MeCP2.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-MeCP2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human MeCP2 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Methyl CpG Binding Protein 2 (MECP2) (AA 7-148) antibody
MECP2 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 8H4A5B9 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 81-170) antibodyMECP2 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1B11 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 1-486) antibodyMECP2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 1-100) antibodyMECP2 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (pSer423) antibodyMECP2 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB29261 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (C-Term) antibodyMECP2 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 400-428) antibodyMECP2 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB25959 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 81-170) antibodyMECP2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4B6 unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (N-Term) antibodyMECP2 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Methyl CpG Binding Protein 2 (MECP2) (AA 1-100) antibodyMECP2 适用: 人 IF 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000 - 1:15,000
Immunohistochemistry_Dilution: 1:200
Western_Blot_Dilution: 1:1000
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说明
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Suggested Applications: This antibody is not suitable for CHIP.
Anti-MeCP2 Antibody has been tested in ELISA, Western Blot, and Immunohistochemistry. Expect a band at ~52 kDa in western blot using appropriate lysates or tissues. Positive control used U2-OS, A431, human cerebellum tissue, and Chicken Spinal Cord in WB, human tonsil tissue in IHC. -
限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- MECP2 (Methyl CpG Binding Protein 2 (MECP2))
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别名
- MECP2
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背景
- Rabbit Anti-Methyl-CpG Binding Protein 2 Antibody, Rabbit-Anti-MeCP2 Antibody, MeCp-2 Protein, Methyl CpG Binding Protein 2 (Rett Syndrome), Testis Tissue Sperm-Binding Protein Li 41a, Mental Retardation, X-Linked 16, Mental Retardation, X-Linked 79, Methyl-CpG-Binding Protein 2, Methyl CpG Binding Protein 2, Rett Syndrome, AUTSX3, MRXS13, MRXSL, MRX16, MRX79, MeCp2, PPMX, RTS, RTT, RS,MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. MECP2 mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. It binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). Anti-MeCP2 Antibody is useful for researchers interested in Rett Syndromes, SIDS, Mental Retardation X-Linked Syndromic 13, and chromatin binding pathways.
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基因ID
- 4204
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NCBI登录号
- NP_001104262
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UniProt
- P51608
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途径
- Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane
抗原
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