MMP2 抗体 (Internal Region)
Quick Overview for MMP2 抗体 (Internal Region) (ABIN7540614)
抗原
See all MMP2 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-mj9s
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Supplier
- Rockland
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原理
- MMP2 Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human MMP2.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-MMP2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human MMP2 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Matrix Metalloproteinase 2 (MMP2) (AA 31-109) antibody
MMP2 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metalloproteinase 2 (MMP2) (AA 40-280) antibodyMMP2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metalloproteinase 2 (MMP2) antibodyMMP2 适用: 人 WB, IP, IF, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metalloproteinase 2 (MMP2) (AA 401-500) antibodyMMP2 适用: 人, 大鼠, 小鼠, 兔 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metalloproteinase 2 (MMP2) (AA 110-660) antibodyMMP2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metalloproteinase 2 (MMP2) (AA 9-140) antibodyMMP2 适用: 人, 小鼠 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 2B11E6 unconjugated
anti-Matrix Metalloproteinase 2 (MMP2) (C-Term) antibodyMMP2 适用: 人, 大鼠, 小鼠, 兔, Cow, 犬, 马, 豚鼠, 绵羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Matrix Metalloproteinase 2 (MMP2) (AA 110-660) antibodyMMP2 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C1 unconjugated
anti-Matrix Metalloproteinase 2 (MMP2) (AA 242-396) antibodyMMP2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4D1E2 unconjugated
anti-Matrix Metalloproteinase 2 (MMP2) (AA 9-140) antibodyMMP2 适用: 人, 小鼠 宿主: 小鼠 Monoclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: 1:100
IF_Microscopy_Dilution: 15 μg/mL
Western_Blot_Dilution: 1:1000
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说明
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Anti-MMP2 Antibody has been tested in ELISA, WB, IF, and IHC. Expect a band at ~ 65, 74kDa in western blot using appropriate lysates. Positive control used: Human placenta and Human lung lysates, as well as HEK293T MMP2 overexpressed lysate in WB, HepG2 and U87MG cells for IF, Human metastatic breast carcinoma in lymph nodes for IHC.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- MMP2 (Matrix Metalloproteinase 2 (MMP2))
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别名
- MMP2
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背景
- Rabbit Anti-MMP2 Antibody, Rabbit Anti-Matrix Metallopeptidase 2 Antibody, Matrix Metalloproteinase-2, CLG4A, MMP-2, TBE-1, Matrix Metalloproteinase 2 Gelatinase A 72 kDa Type IV Collagenase), Matrix Metallopeptidase 2 Gelatinase A 72 kDa Type IV Collagenase), Matrix Metalloproteinase-II, 72 KDa Type IV Collagenase, Collagenase Type IV-A, Neutrophil Gelatinase, 72 KDa Gelatinase, Gelatinase A, EC 3.4.24.24, MMP-II, MONA, CLG4,Matrix metalloproteases (matrix metalloproteinase, MMPs), also called matrixins, are zinc-dependent endopeptidases and the major proteases in ECM degradation. MMPs can degrade several extracellular molecules and several bioactive molecules. MMP2 is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellularly by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, metastasis, tissue repair, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, it can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. MMP2 also cleaves KISS at a Gly- -Leu bond. It appears to have a role in myocardial cell death pathways and contributes to myocardial oxidative stress by regulating the activity of GSK3beta. It cleaves GSK3beta in vitro and is involved in the formation of the fibrovascular tissues in association with MMP14. PEX, the C-terminal non-catalytic fragment of MMP2, possesses anti-angiogenic and anti-tumor properties and inhibits cell migration and cell adhesion to FGF2 and vitronectin. MMP2 is a ligand for integrin/beta3 on the surface of blood vessels. Anti-MMP2 antibody is useful for researchers interested in osteolysis, nodulosis, arthropathy, Apoptosis Research, and Cytokines & Growth Factor Antibodies.
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基因ID
- 4313
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NCBI登录号
- NP_001121363
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UniProt
- P08253
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途径
- Activation of Innate immune Response
抗原
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