SMO 抗体 (Internal Region)
Quick Overview for SMO 抗体 (Internal Region) (ABIN7540600)
抗原
See all SMO 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-mg0s
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Supplier
- Rockland
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原理
- Smoothened Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human SMO.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-Smoothened antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Smoothened conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Smoothened Homolog (Drosophila) (SMO) (AA 551-650) antibody
SMO 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (AA 554-787) antibodySMO 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (AA 28-233) antibodySMO 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) antibodySMO 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3D10 unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (1st Extracellular Domain) antibodySMO 适用: 人, 小鼠 IHC, IHC (p), IF 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (N-Term) antibodySMO 适用: 人, 小鼠, 大鼠, 犬, Bat, Cow, Hamster, 马, 猴, Pig, 兔 WB, IHC, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (AA 68-117) antibodySMO 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (AA 653-787) antibodySMO 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1D9 unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (N-Term) antibodySMO 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Smoothened Homolog (Drosophila) (SMO) (N-Term) antibodySMO 适用: 人, 小鼠, 大鼠 WB, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: 1:100
Western_Blot_Dilution: 1 μg/mL
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说明
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Anti-Smoothened Antibody has been tested in ELISA, WB, and IHC. Expect a band at ~86.4kDa in western blot using appropriate lysates and tissues. Positive control used: Overexpressing lysate and HEK293T in Western Blot, and Human Lung Tissue in Immunohistochemistry.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- SMO (Smoothened Homolog (Drosophila) (SMO))
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别名
- SMO
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背景
- Rabbit Anti-SMO Antibody, Smoothened Frizzled Class Receptor, Smoothened Seven Transmembrane Spanning Receptor, Smoothened Frizzled Family Receptor, Frizzled Family Member 11, Protein GX, SMOH, Seven Transmembrane Helix Receptor, Smoothened (Drosophila) Homolog, Smoothened Homolog (Drosophila), Smoothened Homolog, FZD11, CRJS, Gx,SMO (Smoothened, Frizzled Class Receptor) receptors are non-classical G protein-coupled receptors that probably associates with the patched protein (PTCH) to transduce the hedgehogs proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). It is required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. And it interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation. Diseases associated with SMO include Curry-Jones Syndrome and Basal Cell Carcinoma. Anti-Smoothened Antibody is useful for researchers interested in Cancer research.
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基因ID
- 6608
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UniProt
- Q99835
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途径
- Hedgehog Signaling
抗原
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