HEY1 抗体 (Internal Region)
Quick Overview for HEY1 抗体 (Internal Region) (ABIN7540592)
抗原
See all HEY1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Internal Region
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Supplier Product No.
- 600-401-hb5s
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Supplier
- Rockland
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原理
- HEY1 Antibody
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交叉反应 (详细)
- This affinity purified antibody is directed against human HEY1.
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纯化方法
- The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-HEY1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human HEY1 conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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应用备注
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ELISA_Dilution: 1:10,000 - 1:50,000
Immunohistochemistry_Dilution: 1:100
Western_Blot_Dilution: 1:1000
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说明
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Anti-HEY1 Antibody has been tested in Western Blot, ELISA, and Immunohistochemistry. Expect a band at ~33 kDa in western blot using appropriate tissues or lysates. Positive control used is a recombinant human Hey1 at 1μg/mL for WB, human cerebellum tissue at 1:100 for IHC.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- HEY1 (Ha-Ry/enhancer-of-Split Related with YRPW Motif 1 (HEY1))
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别名
- HEY1
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背景
- Rabbit Anti-HEY1 Antibody, BHLHB31, CHF2, HERP2, HESR1, HRT1, Hairy/Enhancer-Of-Split Related With YRPW Motif 1, Hes Related Family BHLH Transcription Factor With YRPW Motif 1, Hairy And Enhancer Of Split-Related Protein 1, Class B Basic Helix-Loop-Helix Protein 31, Cardiovascular Helix-Loop-Helix Factor 2, Hairy-Related Transcription Factor 1, HES-Related Repressor Protein 1, HRT-1, HESR-1, CHF-2, OAF1,HEY1 belongs to the hairy and enhancer of split-related family (HESR), a transcriptional repressor family. This nuclear protein is induced by the Notch pathway and is mainly associated with cardiovascular development. This family is known to repress GATA4, GATA6, MASH1, and NEUROD4 downstream effects. It's role in liver cancer cell self-renewal is still being studied, as it's suspected role in neurogenesis in mice. Hey1 is associated with diseases such as mesenchymal and periosteal chondrosarcoma. Anti-HEY1 antibody is useful for researchers interested in cancer research, neuroscience research, and Notch signalling.
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基因ID
- 23462
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NCBI登录号
- NP_001035798
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UniProt
- Q9Y5J3
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途径
- Tube Formation
抗原
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