EIF2A 抗体 (N-Term)
Quick Overview for EIF2A 抗体 (N-Term) (ABIN7540570)
抗原
See all EIF2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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Supplier Product No.
- 600-101-ml7s
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Supplier
- Rockland
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原理
- EIF2A Antibody
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交叉反应 （详细）
- This affinity purified antibody is directed against human EIF2A.
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纯化方法
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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过滤
- Sterile filtered
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免疫原
- Anti-EIF2A antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human EIF2A conjugated to Keyhole Limpet Hemocyanin (KLH).
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亚型
- IgG
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anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 448-576) antibody
EIF2A 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3A7A8 unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 448-576) antibodyEIF2A 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 3A7B11 unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) antibodyEIF2A 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 437-465) antibodyEIF2A 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) antibodyEIF2A 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (AA 1-100) antibodyEIF2A 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3D5 unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (Center) antibodyEIF2A 适用: 人, 小鼠, 大鼠 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (pSer52) antibodyEIF2A 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (pSer51) antibodyEIF2A 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A) (Ser326) antibodyEIF2A 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA_Dilution: 1:10,000-1:50,000
IF_Microscopy_Dilution: 5 μg/mL
Western_Blot_Dilution: 1:1000
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说明
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Anti-EIF2A Antibody has been tested in ELISA, WB, and IF. Expect a band at ~65kDa in western blot using appropriate tissues and lysates. Positive control used: HeLa in WB and IF.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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有效期
- 12 months
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- EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))
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别名
- EIF2A
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背景
- Goat Anti-Eukaryotic Translation Initiation Factor 2A Antibody, Eukaryotic Translation Initiation Factor 2A 65 kDa, 65 KDa Eukaryotic Translation Initiation Factor 2A, EIF-2A, MSTP004, MSTP089, MST089, CDA02,EIF2A (Eukaryotic Translation Initiation Factor 2A) functions in the early steps of protein synthesis of a small number of specific mRNAs. It acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. Not be confused with eIF2-alpha (EIF2S1), the alpha subunit of the eIF2 translation initiation complex. The eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner, whereas in contrast, EIF2A binds methionyl-tRNAi to 40S subunits in a codon-dependent manner. Goat Anti-EIF2A Antibody is useful for researchers interested in phototoxic stress, translation initiation factor activity, and ribosome binding.
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基因ID
- 83939
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NCBI登录号
- NP_001305972
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UniProt
- Q9BY44
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途径
- Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
抗原
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