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EIF2A 抗体 (N-Term)

This anti-EIF2A antibody is a 山羊 多克隆 antibody detecting EIF2A in WB, ELISA, IF 和 FM. Suitable for 人.
Rockland
产品编号 ABIN7540569
Supplier Product No.: 600-101-ml7
发货至: 中国

Quick Overview for EIF2A 抗体 (N-Term) (ABIN7540569)

抗原

See all EIF2A 抗体
EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

适用

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宿主

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山羊

克隆类型

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多克隆

标记

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This EIF2A antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)
  • 抗原表位

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    N-Term

    Supplier Product No.

    600-101-ml7

    Supplier

    Rockland

    原理

    EIF2A Antibody

    交叉反应 (详细)

    This affinity purified antibody is directed against human EIF2A.

    纯化方法

    The product was affinity purified from monospecific antiserum by immunoaffinity purification.

    过滤

    Sterile filtered

    免疫原

    Anti-EIF2A antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human EIF2A conjugated to Keyhole Limpet Hemocyanin (KLH).

    亚型

    IgG
  • 应用备注

    ELISA_Dilution: 1:10,000-1:50,000

    IF_Microscopy_Dilution: 5 μg/mL

    Western_Blot_Dilution: 1:1000

    说明

    Anti-EIF2A Antibody has been tested in ELISA, WB, and IF.  Expect a band at ~65kDa in western blot using appropriate tissues and lysates.  Positive control used: HeLa in WB and IF.

    限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

    有效期

    12 months
  • 抗原

    EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

    别名

    EIF2A

    背景

    Goat Anti-Eukaryotic Translation Initiation Factor 2A Antibody, Eukaryotic Translation Initiation Factor 2A 65 kDa, 65 KDa Eukaryotic Translation Initiation Factor 2A, EIF-2A, MSTP004, MSTP089, MST089, CDA02,EIF2A (Eukaryotic Translation Initiation Factor 2A) functions in the early steps of protein synthesis of a small number of specific mRNAs. It acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. Not be confused with eIF2-alpha (EIF2S1), the alpha subunit of the eIF2 translation initiation complex. The eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner, whereas in contrast, EIF2A binds methionyl-tRNAi to 40S subunits in a codon-dependent manner. Goat Anti-EIF2A Antibody is useful for researchers interested in phototoxic stress, translation initiation factor activity, and ribosome binding.

    基因ID

    83939

    NCBI登录号

    NP_001305972

    UniProt

    Q9BY44

    途径

    Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
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