5-Carboxylcytosine 抗体
The 兔 多克隆 anti-5-Carboxylcytosine antibody (ABIN7540519) specifically detects 5-Carboxylcytosine in DB, IF, IP 和 MA.
产品编号 ABIN7540519
Supplier Product No.: 200-401-x50
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for 5-Carboxylcytosine 抗体 (ABIN7540519)
抗原
5-Carboxylcytosine
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Dot Blot (DB), Immunofluorescence (IF), Immunoprecipitation (IP), Multiplex Assay (MA)
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Supplier Product No.
- 200-401-x50
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Supplier
- Rockland
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原理
- 5-caC Antibody
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交叉反应 （详细）
- Cross reactivity with other species was not tested but a wide range is presumed.
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纯化方法
- Anti-5caC Antibody was purified by Protein A purification.
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过滤
- Sterile filtered
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免疫原
- Anti-5caC Antibody was produced in rabbits by repeated immunizations with 5-carboxylcytosine (5caC).
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亚型
- IgG
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anti-5-Carboxylcytosine antibody
DB, IF, ICC, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optional[Neutralization_Dilution]: Dot Blot: 1:500 - 1:1,000
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说明
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Anti-5caC Antibody is tested for Dot Blots, Immunofluorescence and Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
Stabilizer: 30 % Glycerol
Preservative: 0.05 % (w/v) Sodium Azide -
储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
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有效期
- 12 months
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- 5-Carboxylcytosine
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物质类
- Chemical
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背景
- 5ca-CMP monophosphate,Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases. Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base. Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. Anti-5caC Antibody is ideal for research in Genetics and Epigenetics.
抗原
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