5-Carboxylcytosine 抗体
Cited in 5+ publications.
The 兔 多克隆 anti-5-Carboxylcytosine antibody (ABIN2668976) specifically detects 5-Carboxylcytosine in DB, IF, ICC, IHC 和 WB.
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(5 references)
产品编号 ABIN2668976
发货至:
中国
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Quick Overview for 5-Carboxylcytosine 抗体 (ABIN2668976)
抗原
5-Carboxylcytosine
宿主
兔
克隆类型
多克隆
标记
非结合性
应用范围
Dot Blot (DB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Western Blotting (WB)
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原理
- 5-Carboxylcytosine (5-caC) antibody (pAb)
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纯化方法
- Unpurified
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免疫原
- This 5-Carboxylcytosine antibody was raised against 5-carboxylcytidine conjugated to KLH and recognizes 5-carboxylcytosine.
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应用备注
- DB: 1:5,000 dilution
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Rabbit serum containing 30 % glycerol and 0.035 % sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage
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储存条件
- -20 °C
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储存方法
- Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.
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有效期
- 24 months
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: "5-Carboxylcytosine levels are elevated in human breast cancers and gliomas." in: Clinical epigenetics, Vol. 7, pp. 88, (2015) (PubMed).
: "Analysis of TET expression/activity and 5mC oxidation during normal and malignant germ cell development." in: PLoS ONE, Vol. 8, Issue 12, pp. e82881, (2014) (PubMed).
: "Structure of a Naegleria Tet-like dioxygenase in complex with 5-methylcytosine DNA." in: Nature, Vol. 506, Issue 7488, pp. 391-5, (2014) (PubMed).
: "Tet-mediated covalent labelling of 5-methylcytosine for its genome-wide detection and sequencing." in: Nature communications, Vol. 4, pp. 1517, (2013) (PubMed).
: "Generation and replication-dependent dilution of 5fC and 5caC during mouse preimplantation development." in: Cell research, Vol. 21, Issue 12, pp. 1670-6, (2011) (PubMed).
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- 5-Carboxylcytosine
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物质类
- Chemical
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背景
- DNA methylation is an epigenetic event in which DNA methyltransferases (DNMTs) catalyze the reaction of a methyl group to the fifth carbon of cytosine in a CpG dinucleotide. This modification helps to control gene expression and is also involved in genomic imprinting, while aberrant DNA methylation is often associated with disease. 5-methylcytosine is a modified base that is found in the DNA of plants and vertebrates. A second type of DNA methylation exists, 5-hydroxymethylcytosine (5-hydroxy methylcytosine, 5-hmC). This results from the enzymatic conversion of 5-methylcytosine into 5-hydroxymethylcytosine by the TET family of cytosine oxygenases. Iterative activity of TET on 5-hydroxymethylcytosine results in the conversion of 5-hmC into 5-formylcytosine and 5-carboxylcytosine. An increase in levels of 5-formylcytosine and 5-carboxylcytosine are detected in the mouse male pronucleus following fertilization, which is gradually diluted by DNA replication.
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基因ID
- 57279
抗原
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