CD15 抗体 (APC)
Quick Overview for CD15 抗体 (APC) (ABIN7540419)
抗原
See all CD15 (FUT4) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Anti-Hu CD15 APC
-
特异性
- Mouse monoclonal antibody W6D3 recognizes CD15 in nonterminal position on extracellular glycoproteins. CD15 (a cell membrane 3-fucosyl-N-acetyllactosamine, 3-FAL) is strongly expressed on granulocytes, monocytes, macrophages, mast cells, it is also present on Langerhans cells and some myeloid precursors cells. This is a non-IgM anti-CD15 antibody.
-
纯化方法
- Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
-
免疫原
- WERI-RB-1 retinoblastoma cell line
-
亚型
- IgG1 kappa
-
-
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 264-497) antibody
FUT4 适用: 人 IHC, WB, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 144-389) antibodyFUT4 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 251-295) antibodyFUT4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p), ELISA, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibodyFUT4 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-158 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 200-300) antibodyFUT4 适用: 人 IHC, IF, WB 宿主: 兔 Polyclonal unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 1-147) antibodyFUT4 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 6C5A3 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) (AA 199-302) antibodyFUT4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 6B11B4 unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibodyFUT4 适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal Leu-M1-same as MMA unconjugated
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (FITC)FUT4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-158 FITC
anti-Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4) antibody (Biotin)FUT4 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-158 Biotin
-
-
应用备注
- Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C
-
储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
-
-
- CD15 (FUT4) (Fucosyltransferase 4 (Alpha (1,3) Fucosyltransferase, Myeloid-Specific) (FUT4))
-
别名
- CD15
-
背景
-
Description: CD15 (Lewis x), also known as stage specific embryonic antigen-1 (SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several structural relatives of Lewis x, e.g. sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.
Other names: Lewis x Blood Group antigen, Le(x), SSEA-1, 3-fucosyl-N-acetyllactosamine
抗原
-
