Osteopontin 抗体
Quick Overview for Osteopontin 抗体 (ABIN7539195)
抗原
See all Osteopontin (SPP1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Osteopontin is a bone matrix protein and a cytokine secreted by activated T cells. Osteopontin (OPN, also designated bone sialoprotein 1, urinary stone protein, spp-1, -1, nephropontin, uropontin) is an extracellular matrix cell adhesion phosphoglycoprotein. OPN is deposited into unmineralized matrix prior to calcification leading to localization at various tissue interfaces including cement lines, lamina limitans, and between collagen fibrils of fully matured hard tissues. While OPN is a major product of osteoblasts, it is also synthesized by brain and kidney cells. OPNs isolated from or secreted by various tissues have molecular weights due to post-translational modifications. OPN functions as a substrate for transglutaminase and is involved in cell adhesion, chemoattraction and immunomodulation.
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交叉反应 （详细）
- Human, Dog, Mink, Mouse, Rabbit, Rat and Turkey.
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纯化方法
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
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免疫原
- Recombinant full-length rat Spp1 protein
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亚型
- IgG1
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anti-Secreted phosphoprotein 1 (SPP1) (AA 17-317) antibody
SPP1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 18-314) antibodySPP1 适用: 猕猴 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 1-300) antibodySPP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F2 unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 167-314) antibodySPP1 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 7C5H12 unconjugated
anti-Secreted phosphoprotein 1 (SPP1) antibodySPP1 适用: 人 WB, ELISA, IHC, IF 宿主: 小鼠 Monoclonal 7C5H12 unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 167-314) antibodySPP1 适用: 人 WB, ELISA, IHC, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 221-280) antibodySPP1 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (C-Term) antibodyVerified SPP1 适用: 人 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (AA 1-300) antibodySPP1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1F5 unconjugated
anti-Secreted phosphoprotein 1 (SPP1) (C-Term) antibodySPP1 适用: 人, 小鼠, 大鼠, 犬, Cow, 豚鼠, 山羊, 绵羊 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Known_Application: Functional Studies, Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Bone. Found in plasma.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))
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别名
- SPP1
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背景
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BNSP, Bone sialoprotein 1 (BSPI/BSP1), Early T lymphocyte activation 1 (ETA1), Nephropontin, immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1 (osteopontin bone sialoprotein I early T lymphocyte activation 1), Secreted phosphoprotein 1 (SPP1), CALPHA1 fusion, Urinary stone protein, Uropontin,Osteopontin (OSP) / Secreted Phosphoprotein 1 (SPP1)
Cellular localisation: Secreted. -
分子量
- 34.9kDa
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基因ID
- 6696, 313
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UniProt
- P10451
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途径
- Regulation of Cell Size
抗原
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