Dysferlin 抗体 (AA 1999-2016)
Quick Overview for Dysferlin 抗体 (AA 1999-2016) (ABIN7538896)
抗原
See all Dysferlin (DYSF) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1999-2016
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原理
- Lyophilized Mouse Monoclonal Antibody Dysferlin
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特异性
- Reactive with the dysferlin molecule in human skeletal muscle. Also present in many non-muscle tissues.
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产品特性
- This product is intended for the qualitative identification by light microscopy of Dysferlin by immunohistochemistry.
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纯化方法
- Tissue culture supernatant
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免疫原
- Synthetic peptide containing amino acids 1999-2016 of the human dysferlin molecule.
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亚型
- IgG1
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anti-Dysferlin (DYSF) (AA 105-278) antibody
DYSF 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Dysferlin (DYSF) (C-Term) antibodyDYSF 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Dysferlin (DYSF) antibodyDYSF 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Dysferlin (DYSF) (AA 1584-1633) antibodyDYSF 适用: 人, 小鼠, 豚鼠, 兔, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Dysferlin (DYSF) (AA 215-264) antibodyDYSF 适用: 人, Cow, 犬, 豚鼠, 兔, Pig, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Dysferlin (DYSF) (AA 1-479) antibodyDYSF 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Dysferlin (DYSF) (C-Term) antibodyDYSF 适用: 人, 小鼠, 大鼠, Cow, 犬, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Dysferlin (DYSF) (Middle Region) antibodyDYSF 适用: 人, 小鼠, 大鼠, Cow, 犬, 豚鼠, 兔, 马, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Immunohistochemistry on frozen sections: Suggested dilution: 1:20-1:40 for 60 minutes at 25°C. This is provided as a guide and users should determine their own optimal working dilutions. NCL-Hamlet requires the sections to be fixed in acetone/ methanol at a ratio of 1:1 for 4 minutes at 25°C prior to incubation with the primary antibody. Immunohistochemistry on paraffin sections. Suggested dilution: 1:20-1:40 for 60 minutes at 25°C. High temperature antigen retrieval using 0.01 M citrate retrieval solution ( pH 6.0) is recommended. This is provided as a guide and users should determine their own optimal working dilutions.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- The user is required to reconstitute the contents of the vial with the correct volume of sterile distilled water as indicated on the vial label.
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浓度
- 180 mg/L
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缓冲液
- This product is a lyophilized tissue culture supernatant containing sodium azide as a preservative.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.
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有效期
- 12 months
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- Dysferlin (DYSF)
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别名
- Dysferlin
抗原
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