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Perforin 1 抗体 (FITC)

The 小鼠 单克隆 anti-Perforin 1 antibody is suitable to detect Perforin 1 in samples from 人 和 Cow. It has been validated for ICFC.
产品编号 ABIN7506004
发货至: 中国
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Quick Overview for Perforin 1 抗体 (FITC) (ABIN7506004)

抗原

See all Perforin 1 (PRF1) 抗体
Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

适用

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人, Cow

宿主

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小鼠

克隆类型

  • 69
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单克隆

标记

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This Perforin 1 antibody is conjugated to FITC

应用范围

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Intracellular Flow Cytometry (ICFC)

克隆位点

DG9
  • 原理

    Anti-Perforin FITC

    特异性

    The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

    无交叉反应

    小鼠

    纯化方法

    Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

    免疫原

    purified granules from human YT lymphoma cell line

    亚型

    IgG2b kappa
  • 应用备注

    Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

    限制

    仅限研究用
  • 缓冲液

    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • 抗原

    Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

    别名

    Perforin

    背景

    Perforin 1,Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.,PRF1, P1, PFP, HPLH2

    基因ID

    5551

    UniProt

    P14222

    途径

    Apoptosis, Caspase Cascade in Apoptosis
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