IgE 抗体 (PE)
Quick Overview for IgE 抗体 (PE) (ABIN7505984)
抗原
See all IgE 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-Hu IgE PE
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特异性
- The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7.325 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).
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纯化方法
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
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免疫原
- Purified human IgE.
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亚型
- IgG1
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anti-IgE antibody
适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 4G7-325 unconjugated
anti-IgE antibody (APC)适用: 人 FACS 宿主: 小鼠 Monoclonal 4H10 APC
anti-IgE antibody (FITC)适用: 人 FACS 宿主: 小鼠 Monoclonal BE5 FITC
anti-IgE antibody (Biotin)适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal BE5 Biotin
anti-IgE antibody (PE)适用: 人 FACS 宿主: 小鼠 Monoclonal BE5 PE
anti-IgE (AA 1-421) antibody适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
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应用备注
- Flow cytometry: Recommended dilution: 1-5 μg/mL.
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限制
- 仅限研究用
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缓冲液
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- IgE
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背景
- Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
抗原
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