Recombinant T Cell Receptor 抗体
Quick Overview for Recombinant T Cell Receptor 抗体 (ABIN7487759)
抗原
抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Anti-TCR [H57-597], Hamster (Armenian) IgG, lambda
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特异性
- MAb H57-597 reacts with surface receptors on all alpha-beta TCR bearing cells, but does not react with receptors on gamma-delta T cells. H57-597 is directed to an epitope within the FG loop of the C region of TCR beta chain.
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产品特性
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Original Species of Ab: Hamster, Armenian
Original Format of Ab: IgG
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纯化方法
- Protein A affinity purified
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免疫原
- Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
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亚型
- IgG lambda
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anti-T Cell Receptor antibody
适用: 人 FACS, IP, ELISA, IHC (fro) 宿主: 小鼠 Monoclonal 11F2 unconjugated
anti-T Cell Receptor antibody适用: 小鼠 FACS, IP, Crys, IF 宿主: 兔 Chimeric Desire-1 unconjugated Recombinant Antibody
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应用备注
- In an immobilised form, this antibody can directly activate T cells bearing alpha-beta TCR to produce IL-2. H57-597 has also been used to construct a Fab fragment which has been complexed with deglycosylated TCRs for their crystallisation for structure determination (Liu 1996). Flow cytometry is another application of this antibody, where H57-597 has been used to analyse TCR-beta expression in different T cell populations (Tsukumo 2006).
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限制
- 仅限研究用
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浓度
- 1 mg/mL
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缓冲液
- PBS with 0.02 % Proclin 300.
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
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- T Cell Receptor
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别名
- TCR
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背景
- TCR-beta, T cell receptor, T-cell receptor, T cell receptor beta chain, TCRB, TRB, TCB1, Ti
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UniProt
- P01852
抗原
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