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Recombinant CD28 抗体

This anti-CD28 antibody is a 兔 Chimeric antibody detecting CD28 in FACS 和 ELISA. Suitable for 人.

产品编号 ABIN7487692

发货至: 中国

Quick Overview for Recombinant CD28 抗体 (ABIN7487692)

抗原

See all CD28 抗体

CD28

抗体类型

Recombinant Antibody

适用

205
107
52
38
22
17
13
12
7
7
7
6
1
1
1
1

人

宿主

164
82
27
12
8
3
2
1
1

兔

克隆类型

Chimeric

标记

This CD28 antibody is un-conjugated

应用范围

217
99
67
50
43
40
37
28
26
21
13
7
6
6
5
5
5
4
4
4
2
2
2
1
1
1
1
1
1

Flow Cytometry (FACS), ELISA

克隆位点

15E8

Discover our top product CD28 Primary Antibody

原理

Anti-CD28 [15E8], Rabbit IgG, kappa

特异性

This antibody binds human CD28, which is found on a sub-population of T cells and activated B cells. The antibody does not react with B cells, granulocytes or monocytes. CD28 is involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival.

产品特性

Original Species of Ab: Mouse
Original Format of Ab: IgG1

纯化方法

Protein A affinity purified

免疫原

The original mouse antibody was generated by immunizing mice with human T lymphocytes.

亚型

IgG kappa
anti-CD28 (CD28) (AA 1-120) antibody
CD28 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IF (p), IHC (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-CD28 (CD28) antibody
CD28 适用: 人, Non-Human Primate FACS, WB, IP, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) antibody (FITC)
CD28 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal CD28-2 FITC

anti-CD28 (CD28) antibody
CD28 适用: 人, Non-Human Primate FACS, WB, IP, Func, IHC (fro), ICC 宿主: 小鼠 Monoclonal CD28-2 unconjugated

anti-CD28 (CD28) (AA 19-152) antibody
CD28 适用: 人 FACS, ELISA 宿主: 兔 Monoclonal DM61 unconjugated Recombinant Antibody

anti-CD28 (CD28) (Internal Region) antibody
Verified CD28 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-CD28 (CD28) antibody
CD28 适用: 大鼠 FACS, IP 宿主: 小鼠 Monoclonal JJ319 unconjugated

anti-CD28 (CD28) antibody
CD28 适用: 人 FACS, IF, Func 宿主: 小鼠 Monoclonal CB28 unconjugated

anti-CD28 (CD28) antibody
CD28 适用: 人, 小鼠, Cow, Pig, 绵羊 FACS, IF, Func 宿主: 小鼠 Monoclonal 204-12 unconjugated

anti-CD28 (CD28) (C-Term) antibody
CD28 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

This antibody works independently of TcR/CD3 and activation of protein kinase C (PKC) for activation of T cells and nehances cytokine secretion (PMID: 1647959). This antibody is capable of stimulating T cells in combination with either murine OKT3 antibody or CDR-mutated OKT3 Fab variants. It was found that cross-linking of specific tumor antigens with the T-cell-associated CD3 and CD28 antigens can increase IL-2 secretion, proliferation and antigen-specific cytotoxicity in resting T cells. This cross-linking can be achieved effectively by bispecific monoclonal antibodies (BiMAb) with specificity for both the tumor antigen and CD3 or CD28 antigen. A combination of bispecific antibodies BiMAb OKT3/HRS-3 with reactivity against both CD3 and Hodgkin's-lymphoma-associated CD30 antigen and BiMAb 15E8/HRS-3 with reactivity to both CD28 and CD30 antigen was able to activate resting T cells, represented by Jurkat cells (CD3+/CD28+) to produce IL-2 (PMID: 7686889). This antibody was also used in another study wherein the combined use of two CD3 x CD19 plus CD28 x CD22 bispecific antibodies induced optimal interleukin 2 secretion by Jurkat T-cell acute lymphocytic leukemia cells in the presence of target B-cells (PMID: 7689932). This antibody also provides a co-stimulatory signal to T cells. The scFv fragment of this antibody induced proliferation of peripheral blood mononuclear cells in the presence of anti-CD3 or anti-CD2 mAb and enhanced anti-tumor activity of anti-MUC1xanti-CD3 bispecific antibody when tested with lymphokine-activated killer cells with T cell phenotype (PMID: 10913626). The humanzied version of this antibody is available on request.

说明

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

限制

仅限研究用
浓度

1 mg/mL

缓冲液

PBS with 0.02 % Proclin 300.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
抗原

CD28

别名

CD28

背景

T-cell-specific surface glycoprotein CD28, TP44

UniProt

P10747

途径

TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response

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