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Recombinant CD163 抗体

This anti-CD163 antibody is a 小鼠 单克隆 antibody detecting CD163 in FACS, ELISA 和 IF. Suitable for Pig.
产品编号 ABIN7487685
发货至: 中国

Quick Overview for Recombinant CD163 抗体 (ABIN7487685)

抗原

See all CD163 抗体
CD163

抗体类型

Recombinant Antibody

适用

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Pig

宿主

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小鼠

克隆类型

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单克隆

标记

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This CD163 antibody is un-conjugated

应用范围

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Flow Cytometry (FACS), ELISA, Immunofluorescence (IF)

克隆位点

2A10
  • 原理

    Anti-CD163 [2A10 (2A10/11)], Mouse IgG1, lambda

    特异性

    This antibody recognizes and binds porcine CD163.

    产品特性

    Original Species of Ab: Mouse

    Original Format of Ab: IgG1

    纯化方法

    Protein A affinity purified

    免疫原

    The original antibody was generated by immunizing BALB/c mice with porcine alveolar macrophages.

    亚型

    IgG1 lambda
  • 应用备注

    This antibody is recommended to be used as a tool in the study of porcine macrophage maturation and differentiation and for determining their heterogeneity both in normal and pathological conditions. This antibody can recognize porcine CD163 in a western blot assay and an immunoprecipitation assay (PMID: 9151393). 2A10 successfully binds PAM cells in a flow cytometric analysis (PMID:23785195). Porcine CD163 has also been analyzed as potential antigen targeting receptors using mouse IgG as surrogate antigens. MAbs to CD163 induced in vitro PBMC proliferation at concentrations 50-80 fold lower than the control mAb when using, as responder cells, cells from pigs immunized with mouse serum IgGs (PMID:21557980). It is also reported that porcine mAb 2A10 antigen is homologous to human CD163 and related to macrophage differentiation (PMID: 10227997). This antibody was also used to study the role of CD163 in the process of infection of porcine monocytes/macrophages by African swine fever virus infection (PMID: 14648288). This mAb was used in a sandwich enzyme-linked immunoassay (ELISA) to measure levels of sCD163 in porcine sera and biological fluids (Pérez et al, 2008).

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS with 0.02 % Proclin 300.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C,-20 °C

    储存方法

    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • 抗原

    CD163

    别名

    CD163

    背景

    M130, sCD163, Scavenger receptor cysteine-rich type 1 protein M130

    UniProt

    Q2VL90
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