E. Coli 抗体 (FITC)
This anti-E. Coli antibody is a 山羊 多克隆 antibody detecting E. Coli in Func. Suitable for E. coli.
Quick Overview for E. Coli 抗体 (FITC) (ABIN7478177)
抗原
E. Coli
适用
E. coli
宿主
山羊
克隆类型
多克隆
标记
This E. Coli antibody is conjugated to FITC
应用范围
Functional Studies (Func)
-
-
特异性
- Many "O" and "K" antigenic serotypes of Escherichia coli
-
交叉反应 （详细）
- Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
-
纯化方法
- This product consists of purified IgG fraction of the neat antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
-
免疫原
- Mixture of E. coli serotypes
-
亚型
- IgG
-
-
-
-
应用备注
-
TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ). -
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- Lot specific
-
缓冲液
- The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.
-
-
- E. Coli
-
物质类
- Bacteria
抗原
-
You are here:
