E. Coli 抗体 (Peroxidase (POD))
适用: E. coli
WB, EIA, IHC
宿主: 山羊
Polyclonal
Peroxidase (POD)
-
- 抗原 See all E. Coli products
- E. Coli
-
适用
- E. coli
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This E. Coli antibody is conjugated to Peroxidase (POD)
-
应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (IHC)
- 特异性
- Many "O" and "K" antigenic serotypes of Escherichia coli
- 交叉反应 （详细）
- Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
- 纯化方法
- This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
- 免疫原
- Mixture of E. coli serotypes
- 亚型
- IgG
-
-
- 应用备注
-
TITER : > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays - 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
- 储存液
- Thimerosal (Merthiolate)
- 注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
-
- 抗原
- E. Coli
- Abstract
- E. Coli 产品
- 物质类
- Bacteria
-
You are here:
