Recombinant Vimentin 抗体 (AA 2-466)
Quick Overview for Recombinant Vimentin 抗体 (AA 2-466) (ABIN7477619)
抗原
See all Vimentin (VIM) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 2-466
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免疫原
- A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Liver: Kupffer cells must show a strong staining and sinusoidal endothelial cells should show an at least a weak staining. Colon: Endothelial and muscle cells of large vessels and stromal cells must show a strong Vimentin staining while dispersed intraepithelial T-cells must show an at least moderate intensity staining.
Negative Control: Liver: Hepatocytes cells must be Vimentin negative. Colon: All epithelial cells must be negative.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Vimentin (VIM)
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别名
- Vimentin
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背景
- VIM,Vimentin antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P08670
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途径
- Caspase Cascade in Apoptosis
抗原
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