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Recombinant Vimentin 抗体 (AA 2-466)

This anti-Vimentin antibody is a 兔 单克隆 antibody detecting Vimentin in IHC. Suitable for 人.
产品编号 ABIN7477619
发货至: 中国

Quick Overview for Recombinant Vimentin 抗体 (AA 2-466) (ABIN7477619)

抗原

See all Vimentin (VIM) 抗体
Vimentin (VIM)

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This Vimentin antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-458R
  • 抗原表位

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    AA 2-466

    免疫原

    A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Liver: Kupffer cells must show a strong staining and sinusoidal endothelial cells should show an at least a weak staining. Colon: Endothelial and muscle cells of large vessels and stromal cells must show a strong Vimentin staining while dispersed intraepithelial T-cells must show an at least moderate intensity staining.

    Negative Control: Liver: Hepatocytes cells must be Vimentin negative. Colon: All epithelial cells must be negative.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    Vimentin (VIM)

    别名

    Vimentin

    背景

    VIM,Vimentin antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P08670

    途径

    Caspase Cascade in Apoptosis
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