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Recombinant Vimentin 抗体 (AA 2-466)

The 兔单克隆 anti-Vimentin antibody is suitable to detect Vimentin in samples from 人. It has been validated for IHC.

IHC

Duodenum mucosa (Recombinant Vimentin 抗体 (AA 2-466))

IHC

Larynx Strong vimentin positivity of the tumor stroma in a squamous cell carcinoma (Recombinant Vimentin 抗体 (AA 2-466))

IHC

3 images

产品编号 ABIN7470690

发货至: 中国

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中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant Vimentin 抗体 (AA 2-466) (ABIN7470690)

抗原

See all Vimentin (VIM) 抗体

Vimentin (VIM)

抗体类型

Recombinant Antibody

适用

人

宿主

兔

克隆类型

单克隆

标记

This Vimentin antibody is un-conjugated

应用范围

Immunohistochemistry (IHC)

克隆位点

458R

Discover our top product VIM Primary Antibody

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AA 2-466

原理

Vimentin antibody (458R)

免疫原

A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

亚型

IgG
anti-Vimentin (VIM) (AA 371-466) antibody
VIM 适用: 人, 大鼠, 小鼠, Pig, Cow, 猴 WB, ELISA, FACS, IHC (p), ICC, IF (p), IHC (fro), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM 适用: 人 WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM 适用: 人, 大鼠, 小鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, 大鼠, 小鼠, Pig, 小鸡 WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 哺乳动物 WB, FACS, IHC (p), ICC 宿主: 小鼠 Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, IF 宿主: 小鼠 Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 41-140) antibody
VIM 适用: 人 WB, ELISA, IF, IP 宿主: 小鼠 Monoclonal 3E9 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人, Pig, Cow, 小鸡, 犬, Cat, 山羊 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal VM452 unconjugated

anti-Vimentin (VIM) antibody
VIM 适用: 人 WB, IHC, StM 宿主: 兔 Monoclonal VIM-1937R unconjugated Recombinant Antibody

应用备注

IHC 1:100-1:200

说明

Positive Control: Liver: Kupffer cells must show a strong staining and sinusoidal endothelial cells should show an at least a weak staining. Colon: Endothelial and muscle cells of large vessels and stromal cells must show a strong Vimentin staining while dispersed intraepithelial T-cells must show an at least moderate intensity staining.
Product Description: Vimentin antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Liver: Hepatocytes cells must be Vimentin negative. Colon: All epithelial cells must be negative.

实验流程

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

限制

仅限研究用
状态

Liquid

储存条件

4 °C,-20 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

有效期

24 months
抗原

Vimentin (VIM)

别名

Vimentin

背景

Gene Alias: VIM

UniProt

P08670

途径

Caspase Cascade in Apoptosis