Uroplakin 1B 抗体 (AA 109-229)
Quick Overview for Uroplakin 1B 抗体 (AA 109-229) (ABIN7477613)
抗原
See all Uroplakin 1B (UPK1B) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 109-229
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免疫原
- Recombinant fragment (around aa 109-229) of human Uroplakin 1B (UPK1B) protein (exact sequence is proprietary)
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亚型
- IgG
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anti-Uroplakin 1B (UPK1B) (AA 109-229) antibody
UPK1B 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal UPK1B-3102 unconjugated
anti-Uroplakin 1B (UPK1B) (AA 109-229) antibodyUPK1B 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal UPK1B-3081 unconjugated
anti-Uroplakin 1B (UPK1B) (AA 150-197) antibodyUPK1B 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Uroplakin 1B (UPK1B) (AA 131-228) antibodyUPK1B 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4F4 unconjugated
anti-Uroplakin 1B (UPK1B) (AA 159-188) antibodyUPK1B 适用: 人, 小鼠 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB24302 unconjugated
anti-Uroplakin 1B (UPK1B) (AA 166-196), (Middle Region) antibodyUPK1B 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Uroplakin 1B (UPK1B) (AA 110-230) antibodyUPK1B 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Urinary bladder: A strong membranous and cytoplasmic UPK1b immunostaining should be seen in the urothelium (the staining can be limited to the top cell layers or equally involve all cell layers).
Negative Control: Colon: UPK1b immunostaining should be absent in all cells of the colon mucosa.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Uroplakin 1B (UPK1B)
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别名
- Uroplakin 1B
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背景
- Tetraspanin-20, Tspan-20, TSPAN20, UP1b, UPIb, UPK1B, Uroplakin-1b,Uroplakin 1B antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- O75841
抗原
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