Recombinant NKX2-1 抗体
Quick Overview for Recombinant NKX2-1 抗体 (ABIN7477609)
抗原
See all NKX2-1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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免疫原
- Recombinant human TTF-1 protein
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Lung: Nuclear TTF1 staining should be strong in pneumocytes and in basal cells of the terminal bronchioles while a weaker, still moderate to strong staining should be seen in columnar epithelial cells of the terminal bronchioles (low expressor control).
Negative Control: Liver: TTF1 staining must not be seen in any cells.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- NKX2-1 (NK2 Homeobox 1 (NKX2-1))
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别名
- TTF-1 / Thyroid Transcription Factor 1 / NKX2-1
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背景
- BCH, Benign chorea, BHC, Homeobox protein Nkx2.1, NK2 homeobox 1, NKX2.1, NKX2A, TEBP, Thyroid nuclear factor-1, Thyroid specific enhancer binding protein, Thyroid transcription factor-1 (TTF-1), Tin man, TITF1, TTF-1,TTF-1 / Thyroid Transcription Factor 1 / NKX2-1 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P43699
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途径
- Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Glucan Metabolic Process, Feeding Behaviour
抗原
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