Recombinant TdT 抗体
Quick Overview for Recombinant TdT 抗体 (ABIN7477595)
抗原
See all TdT (DNTT) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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亚型
- IgG
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应用备注
- IHC 1:75-1:150
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说明
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Positive Control: Thymus: At least a moderate nuclear immunostaining should be seen in virtually all cortical lymphocytes of the normal thymus.
Negative Control: Thymus: The vast majority of the medullary lymphocytes of the normal thymus should be negative. , Tonsil: The vast majority of lymphoid cells and all epithelial cells should be negative.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))
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别名
- TdT
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背景
- Deoxynucleotidyltransferase terminal, DNTT, Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase, TDT, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal deoxyribonucleotidyltransferase,TdT antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P04053
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途径
- DNA Damage Repair
抗原
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