Recombinant PD-L1 抗体
Quick Overview for Recombinant PD-L1 抗体 (ABIN7477557)
抗原
See all PD-L1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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免疫原
- KLH-conjugated linear peptide corresponding to human PD-L1
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Alveolar macrophages, tonsil crypt epithelium and the placental cytotrophoblast show strong, predominantly membranous positivity. Follicle centre macrophages should show at least moderate intensity staining.
Negative Control: Appendix (negative staining of all epithelial cells)
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- PD-L1 (CD274 (PD-L1))
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别名
- PD-L1
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背景
- B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1,PD-L1 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- Q9NZQ7
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途径
- Cancer Immune Checkpoints
抗原
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