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Occludin 抗体 (AA 282-415)

This anti-Occludin antibody is a 小鼠 单克隆 antibody detecting Occludin in IHC. Suitable for 人.
产品编号 ABIN7477543
发货至: 中国

Quick Overview for Occludin 抗体 (AA 282-415) (ABIN7477543)

抗原

See all Occludin (OCLN) 抗体
Occludin (OCLN)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Occludin antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-415M
  • 抗原表位

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    AA 282-415

    免疫原

    ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.

    Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    Occludin (OCLN)

    别名

    Occludin

    背景

    BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin,Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    Q16625

    途径

    Cell-Cell Junction Organization, Hepatitis C
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