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Occludin 抗体 (AA 282-415)

The 小鼠单克隆 anti-Occludin antibody (Clone 415M) (ABIN7470652) specifically detects Occludin in IHC. The antibody is reactive with 人 samples.

Membranous occludin immunostaining of respiratory epithelium scaled (Occludin 抗体 (AA 282-415))

Clear cell ovarian carcinoma with moderate occludin staining of tumor cells (Occludin 抗体 (AA 282-415))

In squamous epithelium occludin positivity is regularly seen but staining is reduced or absent in basal and superficial cell layers scaled (Occludin 抗体 (AA 282-415))

3 images

产品编号 ABIN7470652

发货至: 中国

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Quick Overview for Occludin 抗体 (AA 282-415) (ABIN7470652)

抗原

See all Occludin (OCLN) 抗体

Occludin (OCLN)

适用

123
72
55
34
18
12
4
3
3
2
2
2

人

宿主

小鼠

克隆类型

单克隆

标记

This Occludin antibody is un-conjugated

应用范围

109
41
37
30
26
26
24
17
13
10
10
6
2
1
1
1
1

Immunohistochemistry (IHC)

克隆位点

415M

Discover our top product OCLN Primary Antibody

抗原表位
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16

12

11

9

6

5

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1
AA 282-415

原理

Occludin antibody (415M)

免疫原

ecombinant fragment of human Occludin protein (around aa 282-415) (exact sequence is proprietary)

亚型

IgG1, kappa
anti-Occludin (OCLN) (AA 431-522) antibody
OCLN 适用: 人, 小鼠, Pig WB, ELISA, FACS, IF (cc), IF (p), IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Occludin (OCLN) (C-Term) antibody
OCLN 适用: 人 WB, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated

anti-Occludin (OCLN) (AA 423-522) antibody
OCLN 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 1G7 unconjugated

anti-Occludin (OCLN) (AA 1-522) antibody
OCLN 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated

anti-Occludin (OCLN) (AA 1-522) antibody
OCLN 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated

anti-Occludin (OCLN) (AA 282-415) antibody
OCLN 适用: 人 WB, IHC (f) 宿主: 小鼠 Monoclonal OCLN-2181 unconjugated

anti-Occludin (OCLN) (AA 350-450) antibody
OCLN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Occludin (OCLN) (AA 285-430) antibody
OCLN 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Occludin (OCLN) (C-Term) antibody
OCLN 适用: 人, 小鼠, 大鼠, 犬 WB, IHC, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal OC-3F10 unconjugated

anti-Occludin (OCLN) (C-Term) antibody
OCLN 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

应用备注

IHC 1:100-1:200

说明

Positive Control: Colon: A strong membranous occludin staining of epithelial cells should be seen with a particularly strong staining at the apical/luminal membranes.
Product Description: Occludin antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: All non-epithelial and non-endothelial cells must not show any occludin staining.

实验流程

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

限制

仅限研究用
状态

Liquid

储存条件

4 °C,-20 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

有效期

24 months
抗原

Occludin (OCLN)

别名

Occludin

背景

Gene Alias: BLCPMG, Occludin, OCLN, Phosphatase 1 regulatory subunit 115, PPP1R115, PTORCH1, Tight junction protein occludin

UniProt

Q16625

途径

Cell-Cell Junction Organization, Hepatitis C

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