Recombinant Nucleolin 抗体 (full length)
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Quick Overview for Recombinant Nucleolin 抗体 (full length) (ABIN7470651)
抗原
See all Nucleolin (NCL) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- full length
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原理
- Nucleolin antibody (623R)
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免疫原
- Recombinant full-length human NCL protein
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: A nuclear nucleolin immunostaining should be seen in all cells. A focus of the staining on nucleoli should be visible - at least - in the superficial epithelial cell layer.
Product Description: Nucleolin antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: A cytoplasmic or membranous nucleolin immunostaining should be absent in all cells.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.
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有效期
- 24 months
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- Nucleolin (NCL)
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别名
- Nucleolin
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背景
- Gene Alias: NCL, Nucl, Nucleolin, Protein C25
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UniProt
- P19338
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途径
- Ribonucleoprotein Complex Subunit Organization
抗原
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