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ENO2/NSE 抗体 (AA 416-433)

This anti-ENO2/NSE antibody is a 小鼠 单克隆 antibody detecting ENO2/NSE in IHC. Suitable for 人.
产品编号 ABIN7477539
发货至: 中国

Quick Overview for ENO2/NSE 抗体 (AA 416-433) (ABIN7477539)

抗原

See all ENO2/NSE (ENO2) 抗体
ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This ENO2/NSE antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-451M
  • 抗原表位

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    AA 416-433

    免疫原

    A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative.

    Negative Control: In the colon, epithelial and lymphatic cells do not show NSE staining.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

    别名

    NSE gamma

    背景

    ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE , -phospho-D-glycerate hydrolyase,NSE gamma antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P09104
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