ENO2/NSE 抗体 (AA 416-433)
Quick Overview for ENO2/NSE 抗体 (AA 416-433) (ABIN7477539)
抗原
See all ENO2/NSE (ENO2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 416-433
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免疫原
- A synthetic peptide of human NSE gamma (around aa416-433) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: In the colon, axons and ganglion cells in lamina propria and muscular wall must show at least a moderate NSE staining, while epithelial and lymphatic cells remain negative.
Negative Control: In the colon, epithelial and lymphatic cells do not show NSE staining.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- NSE gamma
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背景
- ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE , -phospho-D-glycerate hydrolyase,NSE gamma antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P09104
抗原
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