Recombinant MSH6 抗体 (AA 1-100)
Quick Overview for Recombinant MSH6 抗体 (AA 1-100) (ABIN7477533)
抗原
See all MSH6 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-100
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免疫原
- Recombinant fragment (around aa 1-100) of human MSH6 protein (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:75-1:150
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说明
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Positive Control: Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells.
Negative Control: Colon adenocarcinoma with previously documented loss of MSH6 expression: Nuclear staining should be absent in cancer cells, while a nuclear staining must be seen in stromal and inflammatory cells.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- MSH6 (MutS Homolog 6 (E. Coli) (MSH6))
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别名
- MSH6
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背景
- DNA mismatch repair protein Msh6, G/T mismatch-binding protein, GTBP, GTMBP, hMSH6, HNPCC5, HSAP, MSH6, mutS (E. coli) homolog 6, MutS alpha 160 kDa subunit, MutS-alpha 160 kDa subunit, p160, Sperm associated protein,MSH6 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P52701
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途径
- DNA Damage Repair, Chromatin Binding, Production of Molecular Mediator of Immune Response
抗原
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