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Recombinant MSH2 抗体

This anti-MSH2 antibody is a 小鼠 单克隆 antibody detecting MSH2 in IHC. Suitable for 人.
产品编号 ABIN7477531
发货至: 中国

Quick Overview for Recombinant MSH2 抗体 (ABIN7477531)

抗原

See all MSH2 抗体
MSH2 (Mismatch Repair Protein 2 (MSH2))

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This MSH2 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-902M
  • 免疫原

    Recombinant full-length human MSH2 protein

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Tonsil: Virtually all mantle zone B-cells must show an at least weak to moderate nuclear staining. A moderate to strong nuclear staining must be seen in the germinal centre B-cells.

    Negative Control: Colon adenocarcinoma with previously documented loss of MSH2 expression: no nuclear staining should be seen in cancer cells, while a nuclear staining must be seen in stromal and inflammatory cells.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    MSH2 (Mismatch Repair Protein 2 (MSH2))

    别名

    MSH2

    背景

    BAT26, COCA1, DNA mismatch repair protein Msh2, FCC1, hMSH2, HNPCC1, LCFS2, MSH2, MutS homolog 2, MutS homolog 2 colon cancer nonpolyposis type 1, MutS protein homolog 2,MSH2 antibody validated for immunohistochemistry on 76 different Normal Tissues

    途径

    DNA Damage Repair, Production of Molecular Mediator of Immune Response
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