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Mesothelin 抗体 (AA 273-407)

This anti-Mesothelin antibody is a 小鼠 单克隆 antibody detecting Mesothelin in IHC. Suitable for 人.
产品编号 ABIN7477521
发货至: 中国

Quick Overview for Mesothelin 抗体 (AA 273-407) (ABIN7477521)

抗原

See all Mesothelin (MSLN) 抗体
Mesothelin (MSLN)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Mesothelin antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-235M
  • 抗原表位

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    AA 273-407

    免疫原

    Recombinant fragment (around aa 273-407) of human Mesothelin (MSLN) protein (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Tonsil: A fraction of squamous epithelial cells of the tonsil crypts should show strong mesothelin immunostaining. Fallopian tube: An at least moderate mesothelin positivity should be seen at the apical membrane of epithelial cells.

    Negative Control: Tonsil: Squamous epithelial cells of the tonsil surface and all lymphocytes must not show mesothelin immunostaining.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    Mesothelin (MSLN)

    别名

    Mesothelin

    背景

    CAK1, Megakaryocyte potentiating factor, Mesothelin, MSLN, SMR, SMRP,Mesothelin antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    Q13421

    途径

    EGFR Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Carbohydrate Homeostasis, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process
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