Recombinant MCM7 抗体 (AA 195-319)
Quick Overview for Recombinant MCM7 抗体 (AA 195-319) (ABIN7477515)
抗原
See all MCM7 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 195-319
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免疫原
- Recombinant human MCM7 protein fragment (aa195-319)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: A strong nuclear MCM7 immunostaining should be seen in virtually all crypt base cells.
Negative Control: Colon: MCM7 immunostaining should be largely absent in surface epithelial cells and in most stroma cells.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- MCM7 (Minichromosome Maintenance Complex Component 7 (MCM7))
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别名
- MCM7
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背景
- CDC47, DNA replication licensing factor MCM7, MCM7 mini chromosome maintenance deficient 7, Minichromosome Maintenance 7, Mini chromosome maintenance protein 7, P1.1-MCM3, P1CDC47, P85MCM, PNAS146,MCM7 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P33993
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途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
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