Ki-67 抗体 (AA 2293-2478)
Quick Overview for Ki-67 抗体 (AA 2293-2478) (ABIN7477505)
抗原
See all Ki-67 (MKI67) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 2293-2478
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免疫原
- Recombinant human Ki67 protein fragment (around aa 2293-2478) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Tonsil (moderate to strong nuclear staining in 80-90% of the germinal centre B-cells and in the vast majority of the suprabasal squamous epithelial cells).
Negative Control: Liver (nuclear staining reaction only in scattered hepatocytes (<1%), cytoplasmic staining is absent or only weak)
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))
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别名
- Ki-67
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背景
- KI-67, Ki67, KI-67 Antigen (KIA), MKI67, Proliferation related Ki-67 antigen,Ki-67 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P46013
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途径
- Glycosaminoglycan Metabolic Process
抗原
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