Recombinant IgD 抗体
Quick Overview for Recombinant IgD 抗体 (ABIN7477499)
抗原
See all IgD 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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免疫原
- Recombinant human IGHD fragment
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亚型
- IgG
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anti-IgD antibody
适用: 人 WB 宿主: 兔 Monoclonal unconjugated
anti-IgD (AA 1-240) antibody适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-IgD (AA 1-576) antibody适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-IgD antibody适用: 人 FACS, IHC, IF, StM 宿主: 小鼠 Monoclonal IgD26 unconjugated
anti-IgD (AA 111-160) antibody适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-IgD antibody (FITC)GMP Grade 适用: 人 FACS 宿主: 小鼠 Monoclonal IA6-2 FITC
anti-IgD antibody (iFluor™647)GMP Grade 适用: 人 FACS 宿主: 小鼠 Monoclonal IA6-2 iFluor™647
anti-IgD antibody (iFluor™700)GMP Grade 适用: 人 FACS 宿主: 小鼠 Monoclonal IA6-2 iFluor™700
anti-IgD antibody (mFluor™450)GMP Grade 适用: 人 FACS 宿主: 小鼠 Monoclonal IA6-2 mFluor™450
anti-IgD (AA 37-64) antibody适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 607CT8-4-4 unconjugated
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Tonsil: A strong IgD staining should be seen in lymphocytes of the mantle zone.
Negative Control: Tonsil: Epithelial cells and most ""non-mantle zone"" lymphocytes should not stain for IgD.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- IgD
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物质类
- Antibody
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背景
- Pivotal molecule in the immune response,IgD antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P01880
抗原
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