Recombinant ErbB2/Her2 抗体 (Extracellular Domain)
Quick Overview for Recombinant ErbB2/Her2 抗体 (Extracellular Domain) (ABIN7477489)
抗原
See all ErbB2/Her2 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Extracellular Domain
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免疫原
- Recombinant protein encoding the extracellular domain of human c-erbB2
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亚型
- IgG
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应用备注
- IHC 1:50-1:200
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说明
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Positive Control: Colon: Breast cancers with HER2 amplification should show strong positivity. Placenta: A faint staining should be seen at the surface membrane of the syncytiotrophoblast.
Negative Control: Breast: Normal epithelial cells must not show HER2 immunostaining.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:50 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- ErbB2/Her2 (Receptor tyrosine-protein kinase erbB-2 (ErbB2/Her2))
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别名
- HER2 / CD340
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背景
- P185, CD340, Verb b2 Erythroblastic Leukemia Viral Oncogene Homolog 2, Neuro/Glioblastoma Derived Oncogene Homolog,HER2 / CD340 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P04626
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途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Skeletal Muscle Fiber Development
抗原
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