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Recombinant Estrogen Receptor alpha 抗体 (AA 495-595)

The 兔 单克隆 anti-Estrogen Receptor alpha antibody (Clone 564R) (ABIN7470616) specifically detects Estrogen Receptor alpha in IHC. The antibody is reactive with 人 samples.
产品编号 ABIN7470616
发货至: 中国
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Quick Overview for Recombinant Estrogen Receptor alpha 抗体 (AA 495-595) (ABIN7470616)

抗原

See all Estrogen Receptor alpha (ESR1) 抗体
Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This Estrogen Receptor alpha antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

564R
  • 抗原表位

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    AA 495-595

    原理

    Estrogen Receptor antibody (564R)

    免疫原

    Recombinant fragment (around aa495-595) of human ER alpha protein (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Uterine cervix: virtually all squamous and endocervical cells must show a moderate to strong nuclear staining. Tonsil: Scattered follicular dendritic cells in germinal centers and some squamous epithelial cells in tonsil crypts must show an at least weak nuclear ER immunostaining.

    Product Description: Estrogen Receptor antibody validated for immunohistochemistry on 76 different Normal Tissues

    Negative Control: Tonsil: B-cells in mantle zones and within germinal centers must be negative.

    实验流程

    Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

    有效期

    24 months
  • 抗原

    Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

    别名

    Estrogen Receptor

    背景

    Gene Alias: Estrogen Receptor alpha delta 4*5,6,7*/654 isoform,Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 3

    UniProt

    P03372

    途径

    Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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