Recombinant Estrogen Receptor alpha 抗体 (AA 495-595)
Quick Overview for Recombinant Estrogen Receptor alpha 抗体 (AA 495-595) (ABIN7477471)
抗原
See all Estrogen Receptor alpha (ESR1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 495-595
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免疫原
- Recombinant fragment (around aa495-595) of human ER alpha protein (exact sequence is proprietary)
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亚型
- IgG
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anti-Estrogen Receptor 1 (ESR1) (AA 129-312) antibody
ESR1 适用: 人 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal ESR1-3557 unconjugated
anti-Estrogen Receptor 1 (ESR1) antibodyESR1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal ESR1-1904 unconjugated
anti-Estrogen Receptor 1 (ESR1) (AA 2-185) antibodyESR1 适用: 人, 大鼠 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 6A6E8 unconjugated
anti-Estrogen Receptor 1 (ESR1) (pSer167) antibodyESR1 适用: 人, 大鼠 ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Estrogen Receptor 1 (ESR1) (AA 2-185) antibodyESR1 适用: 人 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal ER506 unconjugated
anti-Estrogen Receptor 1 (ESR1) (C-Term) antibodyESR1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Estrogen Receptor 1 (ESR1) (AA 246-273) antibodyESR1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB33807 unconjugated
anti-Estrogen Receptor 1 (ESR1) (AA 246-273) antibodyESR1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Estrogen Receptor 1 (ESR1) (AA 331-360) antibodyESR1 适用: 人, 大鼠 WB, ELISA, FACS, IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Estrogen Receptor 1 (ESR1) (pSer118) antibodyESR1 适用: 人, 小鼠, 大鼠 WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Uterine cervix: virtually all squamous and endocervical cells must show a moderate to strong nuclear staining. Tonsil: Scattered follicular dendritic cells in germinal centers and some squamous epithelial cells in tonsil crypts must show an at least weak nuclear ER immunostaining.
Negative Control: Tonsil: B-cells in mantle zones and within germinal centers must be negative.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))
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别名
- Estrogen Receptor
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背景
- Estrogen Receptor alpha delta 4*5,6,7*/654 isoform,Estrogen Receptor alpha delta 4 +49 isoform, Nuclear receptor subfamily 3 group A member 1,Estrogen Receptor antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P03372
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途径
- Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
抗原
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