Recombinant pan Keratin 抗体
Quick Overview for Recombinant pan Keratin 抗体 (ABIN7477453)
抗原
See all pan Keratin (panKRT) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Cytokeratin, Pan antibody (000R)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Liver: A strong staining of all bile duct epithelial cells and an at least moderate, predominantly membranous staining of most hepatocytes should be seen.
Product Description/Background: Cytokeratin, Pan antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Tonsil: All lymphocytes must not stain. However, interstitial reticulum cells with dendritic/reticular pattern show a weak to moderate fibrillar staining.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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有效期
- 24 months
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