Recombinant KRT20 抗体 (AA 196-323)
Quick Overview for Recombinant KRT20 抗体 (AA 196-323) (ABIN7477451)
抗原
See all KRT20 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 196-323
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免疫原
- Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon mucosa: Staining should be strong at the surface and at least moderate at the basis of krypts.
Negative Control: Tonsil: All cells must stain negative.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- KRT20 (Keratin 20 (KRT20))
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别名
- Cytokeratin 20
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背景
- CK20, Cytokeratin-20, K20, KA20, Keratin 20, keratin 20, type I, Keratin type I cytoskeletal 20, Keratin-20, KRT20,Cytokeratin 20 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P35900
抗原
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