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Recombinant KRT20 抗体 (AA 196-323)

This anti-KRT20 antibody is a 兔 单克隆 antibody detecting KRT20 in IHC. Suitable for 人.
产品编号 ABIN7477451
发货至: 中国

Quick Overview for Recombinant KRT20 抗体 (AA 196-323) (ABIN7477451)

抗原

See all KRT20 抗体
KRT20 (Keratin 20 (KRT20))

抗体类型

Recombinant Antibody

适用

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宿主

  • 83
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克隆类型

  • 101
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单克隆

标记

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This KRT20 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-620R
  • 抗原表位

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    AA 196-323

    免疫原

    Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Colon mucosa: Staining should be strong at the surface and at least moderate at the basis of krypts.

    Negative Control: Tonsil: All cells must stain negative.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    KRT20 (Keratin 20 (KRT20))

    别名

    Cytokeratin 20

    背景

    CK20, Cytokeratin-20, K20, KA20, Keratin 20, keratin 20, type I, Keratin type I cytoskeletal 20, Keratin-20, KRT20,Cytokeratin 20 antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P35900
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