Recombinant Keratin 10 抗体
Quick Overview for Recombinant Keratin 10 抗体 (ABIN7477439)
抗原
See all Keratin 10 (KRT10) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
免疫原
- Recombinant full-lenth human KRT10 protein.
-
亚型
- IgG
-
-
-
-
应用备注
- IHC 1:100-1:200
-
说明
-
Positive Control: Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining.
Negative Control: Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining.
-
实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- Keratin 10 (KRT10)
-
别名
- Cytokeratin 10
-
背景
- BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10,Cytokeratin 10 antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P13645
抗原
-