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Recombinant Keratin 10 抗体

This anti-Keratin 10 antibody is a 小鼠 单克隆 antibody detecting Keratin 10 in IHC. Suitable for 人.
产品编号 ABIN7477439
发货至: 中国

Quick Overview for Recombinant Keratin 10 抗体 (ABIN7477439)

抗原

See all Keratin 10 (KRT10) 抗体
Keratin 10 (KRT10)

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Keratin 10 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-610M
  • 免疫原

    Recombinant full-lenth human KRT10 protein.

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Skin: All squamous epithelial cells (except the basal cell layer) should show strong cytoplasmic staining, while the basal cell layer remains KRT10 negative or shows only minimal staining.

    Negative Control: Colon: All epithelial, inflammatory, stromal and muscle cells must not show any KRT10 staining.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    Keratin 10 (KRT10)

    别名

    Cytokeratin 10

    背景

    BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10,Cytokeratin 10 antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P13645
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