Recombinant Cytokeratin 7 抗体 (AA 1-100)
Quick Overview for Recombinant Cytokeratin 7 抗体 (AA 1-100) (ABIN7477437)
抗原
See all Cytokeratin 7 (KRT7) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
抗原表位
- AA 1-100
-
免疫原
- Recombinant fragment (around aa1-100) of human KRT7 protein (exact sequence is proprietary)
-
亚型
- IgG
-
-
-
-
应用备注
- IHC 1:100-1:200
-
说明
-
Positive Control: Pancreas: CK7 staining is at least weak to moderate cytoplasmic intercalating ducts and strong in large pancreatic ducts.
Negative Control: Pancreas: CK7 staining should be absent in all acinar cells.
-
实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
-
-
- Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
-
别名
- Cytokeratin 7
-
背景
- CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7,Cytokeratin 7 antibody validated for immunohistochemistry on 76 different Normal Tissues
-
UniProt
- P08729
抗原
-