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Recombinant Cytokeratin 5 抗体 (AA 316-590)

This anti-Cytokeratin 5 antibody is a 小鼠 单克隆 antibody detecting Cytokeratin 5 in IHC. Suitable for 人.
产品编号 ABIN7477433
发货至: 中国

Quick Overview for Recombinant Cytokeratin 5 抗体 (AA 316-590) (ABIN7477433)

抗原

See all Cytokeratin 5 (KRT5) 抗体
Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

抗体类型

Recombinant Antibody

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Cytokeratin 5 antibody is un-conjugated

应用范围

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Immunohistochemistry (IHC)

克隆位点

MSVA-605M
  • 抗原表位

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    AA 316-590

    免疫原

    Recombinant fragment (around aa 316-590) of human Cytokeratin 5 (KRT5) protein (exact sequence is proprietary)

    亚型

    IgG
  • 应用备注

    IHC 1:100-1:200

    说明

    Positive Control: Tonsil: A moderate to strong cytoplasmic KRT5 staining should be seen in virtually all squamous epithelial cells. Prostate: A strong cytoplasmic staining should be present in the majority of basal cells.

    Negative Control: Tonsil: Lymphatic cells must not show any KRT5 positivity.

    实验流程

    Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

    限制

    仅限研究用
  • 状态

    Liquid

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
  • 抗原

    Cytokeratin 5 (KRT5) (Keratin 5 (KRT5))

    别名

    Cytokeratin 5

    背景

    58 kDa Cytokeratin, CK5, Cytokeratin-5, DDD1, Epidermolysis Bullosa Simplex 2 (EBS2), Keratin 5, Keratin, Type II Cytoskeletal 5, Keratin-5, KRT5, Type-II Cytoskeletal 5, Type-II keratin Kb5,Cytokeratin 5 antibody validated for immunohistochemistry on 76 different Normal Tissues

    UniProt

    P13647
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