Recombinant CD20 抗体
Quick Overview for Recombinant CD20 抗体 (ABIN7477401)
抗原
See all CD20 (MS4A1) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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免疫原
- Recombinant human full-length MS4A1 protein
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Tonsil or appendix: A strong, predominantly membranous staining of the mantle zone B-cells, the germinal centre B- cells and the interfollicular B-cells is required, while all other cells should stain negative.
Negative Control: Tonsil or appendix: A large fraction of the interfollicular lymphocytes and all epithelial cells must not show any staining.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))
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别名
- CD20
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背景
- APY, ATOPY, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IGER, IGHER, LEU16, Leukocyte surface antigen Leu-16, Ly44, MS4A1, MS4A2,CD20 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P11836
抗原
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