Recombinant CD8 抗体 (AA 135-235)
Quick Overview for Recombinant CD8 抗体 (AA 135-235) (ABIN7477399)
抗原
See all CD8 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 135-235
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免疫原
- Synthetic peptide corresponding to CD8α residues within aa135-235 of CD8α
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亚型
- IgG
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anti-CD8 (CD8) (AA 51-150) antibody
CD8 适用: 人, 小鼠, 大鼠 FACS, IHC (fro), WB, ELISA, IHC (p), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-CD8 (CD8) antibody (PE)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PE
anti-CD8 (CD8) (AA 22-187) antibodyCD8 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-CD8 (CD8) antibodyCD8 适用: 人 IHC, IP, WB, ELISA 宿主: 兔 Monoclonal 3C3 unconjugated Recombinant Antibody
anti-CD8 (CD8) antibodyCD8 适用: 人 FACS, IP, MS 宿主: 小鼠 Monoclonal MEM-31 unconjugated
anti-CD8 (CD8) antibody (Biotin)CD8 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-31 Biotin
anti-CD8 (CD8) antibody (FITC)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 FITC
anti-CD8 (CD8) antibody (PE)CD8 适用: Cat FACS 宿主: 小鼠 Monoclonal fCD8 PE
anti-CD8 (CD8) antibody (PerCP)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 PerCP
anti-CD8 (CD8) antibody (APC)CD8 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-31 APC
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Tonsil (interfollicular areas)
Negative Control: Epithelial tissues, majority of cells in germinal centres of lymphatic organs
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- CD8
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别名
- CD8
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背景
- CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain,CD8 antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P01732
抗原
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