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Recombinant CD4 抗体 (AA 216-396)

This anti-CD4 antibody is a 兔单克隆 antibody detecting CD4 in IHC. Suitable for 人.

$In tonsil tissue a strong CD4 immunostaining is seen in a fraction of T cells and in Langerhans cells of the tonsil surface epithelium. (Recombinant CD4 抗体 (AA 216-396))$ IHC

B CLL containing numerous CD4 positive lymphocytes (Recombinant CD4 抗体 (AA 216-396))

liver (Recombinant CD4 抗体 (AA 216-396))

3 images

产品编号 ABIN7477393

发货至: 中国

Quick Overview for Recombinant CD4 抗体 (AA 216-396) (ABIN7477393)

抗原

See all CD4 抗体

CD4

抗体类型

Recombinant Antibody

适用

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人

宿主

427
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兔

克隆类型

单克隆

标记

This CD4 antibody is un-conjugated

应用范围

Immunohistochemistry (IHC)

克隆位点

MSVA-004R

Discover our top product CD4 Primary Antibody

抗原表位
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AA 216-396

免疫原

Recombinant fragment (around aa 216-396) of human CD4 protein (exact sequence is proprietary)

亚型

IgG
anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 犬, Pig, 兔, Cat, Cow, 绵羊, 豚鼠, 山羊, 马, Avian, 驴, Hamster WB, IHC 宿主: 山羊 Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 适用: 人 FACS, WB, MS 宿主: 小鼠 Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC

anti-CD4 (CD4) antibody (PE)
CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE

anti-CD4 (CD4) (AA 26-200) antibody
CD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

应用备注

IHC 1:100-1:200

说明

Positive Control: Tonsil: A moderate to strong membranous CD4 immunostaining should be seen in a subset of T-lymphocytes which is predominantly located in the interfollicular area. Macrophages in the germinal centres should show a moderate CD4 positivity. Liver: Kupffer cells and endothelial cells in the liver sinusoids must show at least a moderate CD4 staining.
Negative Control: Tonsil: B-cells and epithelial cells must not show any CD4 staining. Liver: Hepatocytes must not show any CD4 staining.

实验流程

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

限制

仅限研究用
状态

Liquid

储存条件

4 °C,-20 °C,-80 °C

储存方法

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
抗原

CD4

别名

CD4

背景

L3T4, Leu3, Ly-4, Lymphocyte antigen CD4, p55, T cell antigen T4/LEU3, T cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4,CD4 antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt

P01730

途径

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

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