Recombinant Androgen Receptor 抗体 (AA 1-100)
Quick Overview for Recombinant Androgen Receptor 抗体 (AA 1-100) (ABIN7477375)
抗原
See all Androgen Receptor (AR) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-100
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免疫原
- Synthetic peptide corresponding to residues (within aa1-100) of Androgen Receptor.
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Kidney: An at least weak to moderate nuclear AR immunostaining should be seen in a subset of kidney tubuli and of glomerular cells.
Negative Control: Colon: AR immunostaining should be absent in colonic mucosa.
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实验流程
- Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
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- Androgen Receptor (AR)
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别名
- Androgen Receptor
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背景
- AIS, Dihydrotestosterone receptor (DHTR), HUMARA, HYSP1, Kennedy disease (KD), Nuclear receptor subfamily 3 group C member 4 (NR3C4), SMAX1, Spinal and bulbar muscular atrophy (SBMA), Testicular Feminization (TFM),Androgen Receptor antibody validated for immunohistochemistry on 76 different Normal Tissues
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UniProt
- P10275
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途径
- Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding
抗原
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